Chang-Tze YUAssociate Professor

Associate Professor, Department of Applied Chemistry, National Chi Nan University 2015-
Assistant Professor, Department of Applied Chemistry, National Chi Nan University 2011-2015
Assistant Professor, Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University 2006-2015
Postdoc Fellow, National Health Research Institutes, Taichung Veterans General Hospital and Yang Ming University 1999-2006
Maser and Ph.D in Genetics, National Yang-Ming University 1991-1999
B.S. in Biology, Tunghai University 1987-1991
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Cancers arise with multiple mechanisms, leading to the difficulty for anticancer therapy. To understand how cancers develop, we firstly focused our study on identifying novel genes with altered expression in cancer tissues. Several genes upregulated or downregulated in lung cancer tissues have been identified and the mechanisms by which they induced cancer formation are partly resolved. Intriguingly, we found that many of those cancer related genes are functionally linked to organelle. For example, the potential oncoprotein HURP regulates the expression of many Golgi assembly factors. Additionally, when HURP is phosphorylated by the oncogenic kinase Aurora-A, the pHURP assembles Golgi ribbon by attracting and stabilizing some cis-Golgi assembly factors, and positions Golgi complex by interacting with nuclear membrane protein Synes.
 
Golgi complex has been pointed out participating in cancer development recently. Firstly, abnormal fragmentation of Golgi complex releases and activates certain oncogenic factors. Secondly, Golgi complex repositions to the cell leading end during cell migration, directionaly provding essential factors for cell movement. We found that HURP, after being methylated by the methyltransferase PRMT5, impaired cell migration via rigidifying Golgi complex; on the contrary, the unmethylated HURP stimulated cell migration by promoting Golgi repositioning via Cdc42-dependent pathway. Thirdly, Golgi complex is fragmented during aggresome formation. Aggresome is a relatively new organelle engaged in quarantining and clearing the misfolded proteins when proteasome fails to remove those protein garbage. We found that aggresome is only generated when Golgi complex is disassembled, thereby releasing some essential factors to the former. Some of those factors enter and enlarge centrosome, and finally aggresome is constructed on the enlarged centrosome. Hence, we are working on how Golgi complex, centrosome and aggresome coordinate each other to ensure the construction of aggresome during protein misfolding pressure, which is highly toxic to cells. Tremendous amounts of misfolded proteins are found in cancer cells, where aggresome is built up to clear those toxic proteins. Inactivation of aggresome is reported providing a alternative way to kill cancer cells. We are on the process testing the cancer cell killing effect of various inhibitors for the aggresome constructing factors released from Golgi complex.


 

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Rsearch grants
26. 解析JMJD6-HURP-NF-B透過組建Golgi ribbon來刺激細胞移行的機制 (Exploring the mechanisms by which The JMJD6-HURP-NF-kB axis promotes cell mihration via assembling Golgi ribbon), MOST 111-2320-B-260-001-，2022/8/1~2023/7/30 (科技部計畫)（主持人）

25. 高基氏體與修飾微管在aggresome形成時的角色分析(The role of Golgi complex and modified tubulins in the formation of aggresome), TCVGH-NCNU1107903，2021/01/01~2021/12/31 (榮暨計畫)（主持人）。

24. 尋找抑制aggresome形成與加強MG132細胞毒性的抗癌藥物 (Screen for the conventional anticancer drugs able to block aggresome formation and enhance MG132-induced cell toxicity)，TCVGH-NCNU1097903，2020/01/01~2020/12/31 (榮暨計畫)（主持人）。
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23. 探討Ajuba/PRMT5/Aurora-A複合體透過修飾HURP與HDAC6調節aggresome結構功能的機制與其毒殺癌細胞的應用潛力(The effects of Ajuba-PRMT5-Aurora-A complex mediated modification of HURP and HDAC6 on the regulation of aggresome formation and its potential application on cancer cell killing)，MOST 108-2320-B-260-001，2019/8/1~2020/7/31 (科技部計畫)（主持人）。
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22. 分析HURP刺激細胞移行的機制 (Exploring the mechanisms by which HURP stimulates cell migration) ，TCVGH-NCNU1087901，2019/01/01~2019/12/31 (榮暨計畫)（主持人）。
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21. 分析具致癌潛力的PRMT5與Aurora-A共同修飾產生Golgi調控因子HURP p725的機制 (Exploring the mechanisms by which the oncogenic PRMT5 and Aurora-A work together to produce the Golgi assembly factor HURP p725)，TCVGH-NCNU1077902，2018/01/01~2018/12/31 (榮暨計畫)（主持人）。
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20. 分析HURP p725與vimentin intermediate filament的相互調控 (The analysis for the interplay of HURP p725 and vimentin intermediate filament)，TCVGH-NCNU1067902，執行期限：2017/01/01~2017/12/31 (榮暨計畫)（主持人）。
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19. 探討細胞如何調和兩種不同複雜的生理網絡: 分析Golgi complex在細胞週期S期細胞快速行進時所扮演的角色 (Exploring how cells harmonize two different complicated biochemical networks: the role of Golgi complex in S phase dependent cell migration)，MOST 105-2320-B-260-001-MY3，執行期限：2016/08/01~2019/07/31 (科技部計畫)（主持人）。
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18. 分析p38/NF-kB和JNK1/2在HURP調節Golgi complex生成中的角色 (Exploring the role p38-NF-kB and JNK1-2 in the HURP-regulated Golgi formation)，TCVGH-NCNU1057905，執行期限：2016/01/01~2016/12/31 (榮暨計畫)（主持人）。
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17. 透過分析HURP p725調控Golgi complex結構之重要性與機制，了解Golgi complex形成新月形的原因 (Dissection of the role of HURP p725 in the assembly of Golgi ribbon)，104-2311-B-260 -001 -，執行期限：2015/08/01~2016/07/31 (科技部計畫)（主持人）。
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16. NF-кB在HURP所調控穩定性微管與高基氏體組裝中的角色 (The role of NF-kB in HURP-regulated formation of stable microtubule and Golgi ribbon), TCVGH-NCNU1047906，執行期限：2015/01/01~2015/12/31 (榮暨計畫)（主持人）。
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15. 穩定性微管在HURP調控高基氏體結構之角色 (The role of stable microtubule in HURP-regulated Golgi assembly)，計畫編號：MOST 103-2311-B-260 -001，執行期限：2014/08/01~2015/07/31(科技部計畫)（主持人）。
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14. NF-κB於Aurora-A/HURP所引發細胞轉型中的角色 (The role of NF-Kb B in Aurora-AHURP cascade-induced cell transformation)，計畫編號： TCVGH-NCNU1037902，執行期限：2014/01/01~2014/12/31 (榮暨計畫)（主持人）。
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13. Aurora-A透過磷酸化Astrin調節細胞分裂期之研究 (Regulation of mitosis progression by Aurora-A-dependent Astrin phosphorylation)，計畫編號：TCVGH-NCNU1027903，執行期限：2013/01/01~2013/12/31 (榮暨計畫)（主持人）。
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12. HURP調控Golgi結構與cyclin E1基因表達之機制 (Exploration of the underlined mechanisms by which HURP regulates Golgi formation and cyclin E1 expression)，計畫編號：101-2311-B-260-001，執行期限：2012/08/01~2013/07/31 (國科會計畫)（主持人）。
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11. PRMT5/HURP促進細胞轉型之研究 (The contribution of PRMT5/HURP cascade to cell transformation)，計畫編號：101DCC0100002，執行期限：2012/01/01~2012/12/31 (榮暨計畫)（主持人）。
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10. Aurora-A/HURP 致細胞轉形機轉之研究 (Exploration of the Aurora-A/HURP- dependent oncogenic pathway)，計畫編號：NSC 98-2320-B-260-001-MY3，執行期限：2009/08/01~2012/07/31(國科會計畫)（主持人）。
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9. 去磷酸化對HURP的調控：PP1α、PP1β、PP1γ以及PP2Cβ對於HURP蛋白質行為功能上的調控 (Regulation of HURP by dephosphorylation: the role of PP1α, PP1β, PP1γ and PP2Cβ in the control of protein behavior and function of HURP)，計畫編號：TCVGH-NCNU 1007902，執行期限：2011/01/01~2011/12/31 (榮暨計畫)（主持人）。
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8. 以修飾聚精胺酸固相萃取技術及質譜技術分析HURP磷酸化位置，計畫編號：99B4，執行期限：2009/01/01 ~ 2011/12/31(大學跨學門科學人才培育銜接計畫)（主持人）。
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7. 發展觀測活體組織及單一細胞基因活性之顯微螢光技術，計畫編號：99-2627-M-260-001，執行期限：2010/08/01~2013/10/31 (跨領域研究計畫)（共同主持人）。
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6. 分析HURP與MEP50交互作用之細胞功能 (The cellular function of the interaction of HURP and MEP50)，計畫編號：TCVGH-NCNU987905，執行期限：2009/01/01~2009/12/31(榮暨計畫)（主持人）。
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5. 透過分析SLAN與Aurora-A的交互調控了解SLAN的分子活性 (Exploring the cellular function of SLAN by studying the interplay of SLAN and Aurora-A)，計畫編號：TCVGH-987324D，執行期限：2009/01/01~2009/12/31 (台中榮民總醫院)（主持人）。
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4. Aurora-A調控HURP控制紡槌絲之研究 (The role of Aurora-A-catalyzed HURP phosphorylation in spindle formation)，計畫編號：TCVGH-NCNU 977901，執行期限：2008/01/01~2008/12/31 (榮暨計畫)（主持人）。
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3. HURP細胞功能之分子機制探討 (The systematic exploration of the molecular activities of HURP)，計畫編號：NSC 96-2320-B-260-001，執行期限：2007/08/01~2008/07/31(國科會計畫)（主持人）。
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2. Aurora-A調控HURP進出細胞核及其對於細胞轉型之研究 (The regulation of HURP nuclear targeting and cell transformation by Aurora-A)，計畫編號：TCVGH-NCNU967906，執行期限：2007/01/01~2007/12/31 (榮暨計畫)（主持人）。
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1. Aurora-A 磷酸化與調節Astrin之研究(Phosphorylation and regulation of Astrin by Aurora-A)，計畫編號： NSC 95-2314-B-260-001，執行期限：2006/10/01~2007/07/31(國科會計畫)（主持人）。
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Google Scholar:
https://scholar.google.com.tw/citations?hl=zh-TW&user=pEHTb18AAAAJ

Selected Publications :
36. HHuang YJ, Chiu SC, Tseng JS, Chen JM, Wei TW, Chu CY, Kao HE, Yang CO, Shih YE, Yang TY, Chiu KY, Teng CJ, Yu CR*. The JMJD6/HURP axis promotes cell migration via NF-κB-dependent centrosome repositioning and Cdc42-mediated Golgi repositioning. J Cell Physiol. 2022 Oct 17. doi: 10.1002/jcp.30900. Epub ahead of print. PMID: 36250981.

35. Chiu SC, Huang YJ, Wei TW, Chen JM, Kuo YC, Huang YJ, Liao YA, Yu CR*. The PRMT5/HURP axis retards Golgi repositioning by stabilizing acetyl-tubulin and Golgi apparatus during cell migration. J Cell Physiol. 2022 Jan;237(1):1033-1043 (*correspondence).

34. Lu WL, Yu CR, Lien HM, Sheu GT, Cherng SH. Cytotoxicity of naringenin induces Bax-mediated mitochondrial apoptosis in human lung adenocarcinoma A549 cells. Environ Toxicol. 2020 Dec;35(12):1386-1394.
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33. Jo-Mei Maureen Chen 1#, Kun-Chieh Chen 2,3#, Shao-Chih Chiu 4,5#, Ru-Yi Chen1#, Jiun-Yi Hsia6,7#,, Yun-Ru Jaoying Huang1, Chang-Tze Ricky Yu1*. Differential contribution of Protein phosphatase 1 alpha to cell transformation of different cell types. Oncology Reports. 2019 Aug. 42: 1598-1608. doi: 10.3892/or.2019.7272. [Epub ahead of print].
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32. Chiu SC, Chen KC, Hsia JY, Chuang CY, Wan CX, Wei TW, Huang YJ, Chen JM, Liao YA, Yu CR*. Overexpression of Aurora-A bypasses cytokinesis through phosphorylation of SLAN. Am J Physiol Cell Physiol. 2019 Sep 1;317(3):C600-C612. doi: 10.1152/ajpcell.00032.2019. Epub 2019 Jul 17. PMID: 31314582.
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31.  H. CHEN*, W. C. WENG, Y. C. CHANG, J. L. CHIU, C. Y. LI, W. M. SU, Y. T. CHEN, C. C. LU, Y. T. A. LIAO, C. T. R. YU, Y. S. LIN. TUNING ZINC OXIDE NANORODS ON SiO2SUBSTRATES BY INCORPORATING GRAPHENE. Digest Journal of Nanomaterials and Biostructures. 2018 Apr-June. 13(2): p375-380.
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30. Fu-Jye Sheu , Chun-Pei Cho * , Yu-Ting Liao and Chang-Tze Yu. Ag3PO4-TiO2-Graphene Oxide Ternary Composites with Efficient Photodegradation, Hydrogen Evolution, and Antibacterial Properties. Catalysts 2018, 8(2), 57; doi:10.3390/catal8020057 (registering DOI)
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29. Tong-You Wade Wei, Pei-Yu Wu, Ting-Jung Wu, Hsin-An Hou, Wen-Chien Chou, Chieh-Lin Jerry Teng, Chih-Ru Lin, Jo-Mei Maureen Chen, Ting-Yang Lin, Hsiang-Chun Su, Chang-Tze Ricky Yu, Shih-Lan Hsu, Hwei-Fang Tien, Ming-Daw Tsai. Identification of TIFA as a novel therapeutic target in acute myeloid leukemia. Cancer Research (2017), 77(2): 494-508.
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28. Chia-Yun Chen*, Li-Jen Hsu, Po-Hsuan Hsiao, Chang-Tze Ricky Yu. SERS detection and antibacterial activity from uniform incorporation of Ag nanoparticles with aligned Si nanowires. Applied Surface Science (2015), Volume 355, 15 November 2015, Pages 197–202.
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27. Jo-Mei Maureen Chen, Shao-Chih Chiu, Tong-You Wade Wei, Shin-Yi Lin, Cheong-Meng Chong, Chi-Chen Wu, Jiao-Ying Huang, Shu-Ting Yang, Chia-Feng Ku, Jiun-Yi Hsia, Chang-Tze Ricky Yu*.The involvement of Nuclear factor-κappaB in the nuclear targeting and cyclin E1 upregulating activities of Hepatoma upregulated protein. Cellular Signalling 2015 Jan;27(1):26-36.
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26. Tong-You Wade Wei*, Jiun-Yi Hsia*, Shao-Chih Chiu*, Li-Jen Su*, Chi-Chang Juan*, Yuan-Chii Gladys Lee*, Jo-Mei Maureen Chen, Hsiang-Yun Chou, Jiao-Ying Huang, Hiang-Ming Huang, Chang-Tze Ricky Yu*. Methylosome protein 50 promotes androgen- and estrogen-independent tumorigenesis. Cellular Signalling 2014 Dec;26(12):2940-50.
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25. Shao-Chih Chiu, Jo-Mei Maureen Chen, Tong-You Wade Wei, Tai-Shan Cheng, Ya-Hui Candice Wang, Chia-Feng Ku, Lian CH, Chiao-Hsuan Lian, Chun-Chih Jared Liu, Yi-Chun Kuo, Chang-Tze Ricky Yu*. The mitosis regulating and protein-protein interacting activates of Astrin are controlled by the Aurora-A-induced phosphorylation. American Journal of Physiology-Cell Physiology 2014 Dec;26(12):2940-50.
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24. Hsiang Chen, Chin-Pang Chen, Chang-Tze Ricky Yu, Yun Ti Chen, Chin-Chou Teng, Kuang-Yu Lo, Chun Han Lin, Bo-Yun Huanga. Distinct  spatial  profiles  and  antibacterial  effects  of  20-nm  Ag nanoparticles  dripped  on  ZnO  nanorods  grown  on  a polished  Ti  substrate. Applied Surface Science 2014 311:422–425.
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23. Teng CL, Yu CT, Hwang WL, Tsai JR, Liu HC, Hwang GY, Hsu SL. Effector mechanisms of sunitinib-induced G1 cell cycle arrest, differentiation, and apoptosis in human acute myeloid leukaemia HL60 and KG-1 cells. Annals of hematology 2013 Mar; 92(3):301-13.
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22. Chun-Chi Wu, Tsung-Ying Yang, Chang-Tze Ricky Yu, Liem Phan, Cristina Ivan, Anil K Sood, Shih-Lan Hsu, Mong-Hong Lee. p53 negatively regulates Aurora A via both transcriptional and posttranslational regulation. Cell Cycle 2012 Sep; 15;11(18):3433-42.
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21. Tong-You Wade Wei, Chi-Chang Juan, Jiun-Yi Hsia, Li-Jen Su, Yuan-Chii Gladys Lee , Hsiang-Yun Chou, Jo-Mei Maureen Chen, Yu-Chung Wu, Shao-Chih Chiu, Chung-Ping Hsu, Kuo-Lin Liu, Chang-Tze Ricky Yu*. PRMT5 overexpressed in lung cancer tissues transforms cells, upregulates G1 phase cyclins/CDKs and activates PI3K/AKT signaling cascades. Cancer Science 2012 Sep; 103(9):1640-1650.
 
20. Chang-Tze Ricky Yu#, Jiun-Yi Hsia#, Yun-Chih Hseih, Li-Jen Su, TC Lee, Ke-Shin Chen, Chia-Feng Ku, Jou-May Maureen Chen, YCG Lee, Chi-Ying F. Huang, Yu-Chung Wu, Tong-You Wade Wei, Shih-Lan Hsu*. The novel protein SLAN downregulated in lung cancer tissues retards cell proliferation and inhibits the oncokinase Aurora-A. Journal of Thoracic Oncology 2011 Jun; 6 (6): 988-997.
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19. Chun Chi Wu, Chang-Tze Ricky Yu, Gee-Chen Chang, Jin-Mei Lai and Shih-Lan Hsu*. Aurora-A promotes gefitinib resistance via a NF-κB signaling pathway in p53 knockdown lung cancer cells. Biochemical and Biophysical Research Communications 2011 Feb; 405 (2): 168-172.
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18. Chia-Man Chou, Tong-You Wade Wei, Jou-May Maureen Chen, Wei-Ting Chang, Chang-Tze Ricky Yu, and Vincent K. S. Hsiao*. Preparation of Nanoporous Polymer Films for Real-Time Viability Monitoring of Cells. Journal of Nanomaterials. 2011; Article ID 436528, 6 pages.
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17. Gee-Chen Chang#, Chang-Tze R. Yu#, Ching-Hwa Tsai, Jia-Rong Tsai, Jen-Chi Chen, Chun-Chi Wu and Shih-Lan Hsu*. An epidermal growth factor inhibitor, Gefitinib, induces apoptosis through a p53-dependent upregulation of pro-apoptotic molecules and downregulation of anti-apoptotic molecules in human lung adenocarcinoma A549 cells. European Journal of Pharmacology 2008 Dec; 600: 37-44.
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16. Yi-Chung Lee, Chang-Tze Ricky Yu, Kon-Ping Lin, Ming-Hon Chang, Shih-Lan Hsu, Ya-Fen Liu, Bing-Wen Soong*. MPZ mutation G123S characterization: Evidence for a complex pathogenesis in CMT disease. Neurology 2008 Jan; 70: 273-277.
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15. Cheng TS, Hsiao YL, Lin CC, Chang-Tze Ricky Yu, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR*. Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin. Journal of Biological Chemistry. 2008 Jan; 283: 2454-2464.
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14.Chang-Tze R. Yu, Jih-Heng Li*, Te-Chang Lee and Lih-Fang Lin. Characterization of cocaine-elicited cell vacuolation: the involvement of calcium/calmodulin in organelle deregulation. Journal of Biomedical Science. 2008 Mar; 15: 215-226.
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13.  Chang-Tze R. Yu, Jiunn-Chyi Wu, Mei-Chih Liao, Shih-Lan Hsu and Chi-Ying F. Huang*. Identification of c-Fos as a mitotic phosphoprotein: regulation of c-Fos by Aurora-A. Journal of Biomedical Science 2008;Jan; 15: 79-87.
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12. Tai-Shan Cheng, Yun-Ling Hsiao, Ching-Chih Lin, Ching-Mei Hsu, Mau-Sun Chang, Chu-I Lee, Chang-Tze R. Yu, Chi-Ying F. Huang, Shen-Long Howng, Yi-Ren Hong*. hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases. Experimental Cell Research. 2007; 313: 1710-1721.
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11. Chang-Tze R. Yu#, Shih-Lan Hsu#, Sui-Chu Yin#, Ming-Jer Tang#, An-Chi Tien, Yi-Mi Wu, and Chi-Ying F. Huang*. Caspase 3, periodically expressed and activated at G2/M transition, is required for nocodazole-induced mitotic checkpoint. Apoptosis. 2006; 11: 765-771.
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10. Chang-Tze R. Yu#, Yong-Shiang Lin#, Li-Jen Su#, Su-Liang Chen, Fen-Hwa Wong, Wey-Jinq Lin, Chiun-Jye Yuan and Chi-Ying F. Huang*. Gene expression profiles of Aurora family kinases. Gene Expression. 2006; 13: 15-26.
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9. Shur-Hueih Cherng, Shih-Lan Hsu, Jia-Ling Yang, Chang-Tze R. Yu, and Huei Lee*. Suppressive Effect of 1-Nitropyrene on Benzo[a]pyrene-Induced CYP1A1 Protein Expression in HepG2 Cells. Toxicology Letters. 2006; 161: 236-243.
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8. Yan-Nian Chen, Chi-Chih Cheng, Chang-Tze R. Yu, Su-Jen Chou, Hong-Chen Chen, and Shih-Lan Hsu*. Involvement of intrinsic and extrinsic apoptotic pathways in Baicalein-induced apoptosis in human hepatoma Hep3B cells. Journal of Cancer Molecules. 2005; 1: 37-45.
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7. Chang-Tze R. Yu, Jung-Mao Hsu, Yuan-Chii Gladys Lee, Ann-Ping Tsou, Chen-Kung Chou and Chi-Ying F. Huang*. Phosphorylation and stabilization of HURP by Aurora-A: the implication of HURP as a transforming target of Aurora-A. Molecular and Cellular Biology. 2005; 25: 5789-5800.
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6. JC Wu, TY Chen, Chang-Tze R. Yu, SJ Tsai, JM Hsu, MJ Tang, CK Chou, WJ Lin, CJ Yuan, CY Huang*. Identification of V23RalA-Ser194 mediates cellular motility and transformation of Aurora-A through small pool expression screening. Journal of Biological Chemistry.2005; 280: 9013-9022.
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5. Jung-Mao Hsu, Yuan-Chii G. Lee, Chang-Tze R. Yu and Chi-Ying F. Huang*. Fbx7 functions in the SCF complex regulating Cdk1-cyclin B- phosphorylated HURP proteolysis by proline-rich region. Journal of Biological Chemistry. 2004; 279: 32592-32602.
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4. Ann-Ping Tsou#, Chu-Wen Yang#, Chi-Ying Huang#, Chang-Tze R. Yu, Yuang-Chii G. Lee, Cha-Wei Chang, Bo-Rue Chen, Yu-Fang Chung, Ming-Ji Fann, Chin-Wen Chi, Jen-Hwey Chiu and Chen-Kung Chou*. Identification of a novel cell cycle regulated gene, HURP, over-expressed in human hepatocellular carcinoma. Oncogene. 2003; 22: 298-307.
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3. Chang-Tze R. Yu, Te-Chang Lee, T. C. Wang and Jih-Heng Li*. Genetic toxicity of cocaine. Carcinogenesis. 1999; 20: 1193-1199.
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2. YH Sun*, CJ Tsai, MM Green, JL Chao, Chang-Tze R. Yu, TJ Jaw, JY Yeh, and VN Bolshakov. White as a reporter gene to detect transcriptional silencers specifying position-specific gene expression during Drosophila melanogaster eye development. Genetics. 1995;141: 1075-1086.
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1. Chang-Tze R. Yu and Y. Henry Sun*. Tilted agarose gel electrophoresis improves resolution. BioTechniques. 1995;19: 574-575.
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執行計畫(Research grants)
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25. 高基氏體與修飾微管在aggresome形成時的角色分析(The role of Golgi complex and modified tubulins in the formation of aggresome), TCVGH-NCNU1107903，2021/01/01~2021/12/31 (榮暨計畫)（主持人）。
 
24. 尋找抑制aggresome形成與加強MG132細胞毒性的抗癌藥物 (Screen for the conventional anticancer drugs able to block aggresome formation and enhance MG132-induced cell toxicity)，TCVGH-NCNU1097903，2020/01/01~2020/12/31 (榮暨計畫)（主持人）。
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23. 探討Ajuba/PRMT5/Aurora-A複合體透過修飾HURP與HDAC6調節aggresome結構功能的機制與其毒殺癌細胞的應用潛力(The effects of Ajuba-PRMT5-Aurora-A complex mediated modification of HURP and HDAC6 on the regulation of aggresome formation and its potential application on cancer cell killing)，MOST 108-2320-B-260-001，2019/8/1~2020/7/31 (科技部計畫)（主持人）。
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22. 分析HURP刺激細胞移行的機制 (Exploring the mechanisms by which HURP stimulates cell migration) ，TCVGH-NCNU1087901，2019/01/01~2019/12/31 (榮暨計畫)（主持人）。
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21. 分析具致癌潛力的PRMT5與Aurora-A共同修飾產生Golgi調控因子HURP p725的機制 (Exploring the mechanisms by which the oncogenic PRMT5 and Aurora-A work together to produce the Golgi assembly factor HURP p725)，TCVGH-NCNU1077902，2018/01/01~2018/12/31 (榮暨計畫)（主持人）。
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20. 分析HURP p725與vimentin intermediate filament的相互調控 (The analysis for the interplay of HURP p725 and vimentin intermediate filament)，TCVGH-NCNU1067902，執行期限：2017/01/01~2017/12/31 (榮暨計畫)（主持人）。
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19. 探討細胞如何調和兩種不同複雜的生理網絡: 分析Golgi complex在細胞週期S期細胞快速行進時所扮演的角色 (Exploring how cells harmonize two different complicated biochemical networks: the role of Golgi complex in S phase dependent cell migration)，MOST 105-2320-B-260-001-MY3，執行期限：2016/08/01~2019/07/31 (科技部計畫)（主持人）。
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18. 分析p38/NF-kB和JNK1/2在HURP調節Golgi complex生成中的角色 (Exploring the role p38-NF-kB and JNK1-2 in the HURP-regulated Golgi formation)，TCVGH-NCNU1057905，執行期限：2016/01/01~2016/12/31 (榮暨計畫)（主持人）。
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17. 透過分析HURP p725調控Golgi complex結構之重要性與機制，了解Golgi complex形成新月形的原因 (Dissection of the role of HURP p725 in the assembly of Golgi ribbon)，104-2311-B-260 -001 -，執行期限：2015/08/01~2016/07/31 (科技部計畫)（主持人）。
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16. NF-кB在HURP所調控穩定性微管與高基氏體組裝中的角色 (The role of NF-kB in HURP-regulated formation of stable microtubule and Golgi ribbon), TCVGH-NCNU1047906，執行期限：2015/01/01~2015/12/31 (榮暨計畫)（主持人）。
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15. 穩定性微管在HURP調控高基氏體結構之角色 (The role of stable microtubule in HURP-regulated Golgi assembly)，計畫編號：MOST 103-2311-B-260 -001，執行期限：2014/08/01~2015/07/31(科技部計畫)（主持人）。
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14. NF-κB於Aurora-A/HURP所引發細胞轉型中的角色 (The role of NF-Kb B in Aurora-AHURP cascade-induced cell transformation)，計畫編號： TCVGH-NCNU1037902，執行期限：2014/01/01~2014/12/31 (榮暨計畫)（主持人）。
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13. Aurora-A透過磷酸化Astrin調節細胞分裂期之研究 (Regulation of mitosis progression by Aurora-A-dependent Astrin phosphorylation)，計畫編號：TCVGH-NCNU1027903，執行期限：2013/01/01~2013/12/31 (榮暨計畫)（主持人）。
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12. HURP調控Golgi結構與cyclin E1基因表達之機制 (Exploration of the underlined mechanisms by which HURP regulates Golgi formation and cyclin E1 expression)，計畫編號：101-2311-B-260-001，執行期限：2012/08/01~2013/07/31 (國科會計畫)（主持人）。
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11. PRMT5/HURP促進細胞轉型之研究 (The contribution of PRMT5/HURP cascade to cell transformation)，計畫編號：101DCC0100002，執行期限：2012/01/01~2012/12/31 (榮暨計畫)（主持人）。
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10. Aurora-A/HURP 致細胞轉形機轉之研究 (Exploration of the Aurora-A/HURP- dependent oncogenic pathway)，計畫編號：NSC 98-2320-B-260-001-MY3，執行期限：2009/08/01~2012/07/31(國科會計畫)（主持人）。
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9. 去磷酸化對HURP的調控：PP1α、PP1β、PP1γ以及PP2Cβ對於HURP蛋白質行為功能上的調控 (Regulation of HURP by dephosphorylation: the role of PP1α, PP1β, PP1γ and PP2Cβ in the control of protein behavior and function of HURP)，計畫編號：TCVGH-NCNU 1007902，執行期限：2011/01/01~2011/12/31 (榮暨計畫)（主持人）。
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8. 以修飾聚精胺酸固相萃取技術及質譜技術分析HURP磷酸化位置，計畫編號：99B4，執行期限：2009/01/01 ~ 2011/12/31(大學跨學門科學人才培育銜接計畫)（主持人）。
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7. 發展觀測活體組織及單一細胞基因活性之顯微螢光技術，計畫編號：99-2627-M-260-001，執行期限：2010/08/01~2013/10/31 (跨領域研究計畫)（共同主持人）。
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6. 分析HURP與MEP50交互作用之細胞功能 (The cellular function of the interaction of HURP and MEP50)，計畫編號：TCVGH-NCNU987905，執行期限：2009/01/01~2009/12/31(榮暨計畫)（主持人）。
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5. 透過分析SLAN與Aurora-A的交互調控了解SLAN的分子活性 (Exploring the cellular function of SLAN by studying the interplay of SLAN and Aurora-A)，計畫編號：TCVGH-987324D，執行期限：2009/01/01~2009/12/31 (台中榮民總醫院)（主持人）。
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4. Aurora-A調控HURP控制紡槌絲之研究 (The role of Aurora-A-catalyzed HURP phosphorylation in spindle formation)，計畫編號：TCVGH-NCNU 977901，執行期限：2008/01/01~2008/12/31 (榮暨計畫)（主持人）。
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3. HURP細胞功能之分子機制探討 (The systematic exploration of the molecular activities of HURP)，計畫編號：NSC 96-2320-B-260-001，執行期限：2007/08/01~2008/07/31(國科會計畫)（主持人）。
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2. Aurora-A調控HURP進出細胞核及其對於細胞轉型之研究 (The regulation of HURP nuclear targeting and cell transformation by Aurora-A)，計畫編號：TCVGH-NCNU967906，執行期限：2007/01/01~2007/12/31 (榮暨計畫)（主持人）。
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1. Aurora-A 磷酸化與調節Astrin之研究(Phosphorylation and regulation of Astrin by Aurora-A)，計畫編號： NSC 95-2314-B-260-001，執行期限：2006/10/01~2007/07/31(國科會計畫)（主持人）。
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